General Instructions

General Instructions

Venipuncture Technique and Specimen Collection:

Select tube or tubes appropriate for required specimen.

Remove the cover over the valve section of the needle.

Thread the needle into the holder. Be sure needle is firmly seated to ensure needle does not unthread during use.

Apply tourniquet (max. 1 minute) Prepare venipuncture site with an appropriate antiseptic.


Place patient's arm in a downward position.

Remove needle shield. Perform venipuncture WITH ARM DOWNWARD AND TUBE CAP UPPER­MOST.

Push tube into the holder and onto the needle valve puncturing the rubber diaphragm. Center tubes in holder when penetrating the cap to prevent sidewall penetration and subsequent premature vacuum loss.

REMOVE TOURNIQUET AS SOON AS BLOOD APPEARS IN TUBE. DO NOT ALLOW CONTENTS OF TUBE TO CONTACT THE CAP OR END OF THE NEEDLE DURING PROCEDURE. Always hold in place by pressing the tube with the thumb to ensure complete vacuum draw.

NOTE: Blood may occasionally leak from the needle sleeve. Practice universal safety precautions to minimise hazard exposure.

When the first tube is full and blood flow ceases, remove it from holder.

If no blood flows into tube or if blood flow ceases before an adequate specimen is collected, the following steps are suggested to complete satisfactory collection:

  1. Push tube forward until tube cap has been penetrated. Always hold in place by pressing the tube with the thumb to ensure complete vacuum draw.
  2. Confirm correct position of needle in vein.
  3. If blood still does not flow, remove tube and place new tube onto the holder.
  4. If even the second tube does not draw, remove needle and discard. Repeat procedure from step 1.


  • Place succeeding tubes in holder, puncturing diaphragm to begin flow. Draw tubes without additives before tubes with additives. See recommended Order of Draw.
  • While each successive tube is filling, turn the filled tube upside­down and return it to upright position. This is one complete inversion. For proper additive performance, gently invert each tube 8 - 10 times.

Do not shake. Vigorous mixing may cause foaming or hemolysis. Insufficient mixing or delayed mixing in serum tubes may result in delayed clotting. In tubes with anticoagulants, inadequate mixing may result in platelet clumping, clotting and/or incorrect test results.

A soon as blood stops flowing in the last tube, remove needle from vein, applying pressure to puncture site with dry sterile swab until bleeding stops.

Once clotting has occurred, apply bandage if desired.

After venipuncture, the top of the cap may contain residual blood. Take proper precautions when handling tubes to avoid contact with this blood. Any needle holder that becomes contaminated with blood is considered hazardous and should be decontaminated with an appropriate disinfectant or disposed of immediately.

 Dispose of the used needle using an appropriate disposal device. DO NOT RECAP. Recapping of needles increases the risk of needle stick injury and blood exposure.

It is the laboratory's ultimate responsibility to verify that a change from one tube to another does not significantly affect analytical results obtained from patient samples.


Ensure that tubes are properly seated in the centrifuge carrier; incomplete seating could result in the separation of the VACUTUBE Safety Stopper from the tube. VACUTUBE Serum Tubes should be centrifuged 30 minutes after blood collection (to ensure blood is clotted).


Tube Type Recommended relative centrifugal force (rcf) Rec. time
VACUTUBE Serum Tubes Minimum 1500 g 10 min
VACUTUBE Serum Tubes with Separator Gel 1800 g 10 min
VACUTUBE Plasma Tubes 2000 - 3000 g 15 min
VACUTUBE Coagulation Tubes
Platelet tests (PRP) 150 g 5 min
Routine tests (PPP) 1500 - 2000 g 10 min
Preparation for deep freeze plasma (PFP) 2500 - 3000 g 20 min

Tubes should not be re­centrifuged once barrier has formed.

To prevent heating during centrifugation, set refrigerated centrifuges to 25°C.

Gel separation tubes should be centrifuged no later than 2 hours after collection.

Stopper safety: The stopper is designed to allow a controlled and safe opening of the test tube, preventing any aerosol formation on removal. Unscrewing is not necessary.